624 research outputs found
Insulin-like growth factor I is an independent coregulatory modulator of natural killer (NK) cell activity.
We aimed to investigate the natural killer (NK) cell activity in
hGH-deficient adults and to analyze the effect of insulin-like growth
factor (IGF)-I in uivo and in vitro on NK cell activity. NK cell activity
was measured in a 4-h nonisotopic assay with europium-labeled and
cryopreserved K-562 cells. NK-cell numbers were measured after
incubation with murine monoclonal CD3 and CD16 antibodies by flow
cytometry analysis. In a cross-sectional study, the basal and interferon-
p (IFN-P) stimulated (1000 IU/ml) NK cell activity of 15 hGHdeficient
patients and 15 age- and sex-matched controls was measured.
The percentages and absolute numbers of CD3./16+ NK-cells
were not significantly different in the patient vs. control group. The
basal and IFN-P stimulated NK cell activity however was significantly
decreased in the patient vs. control group at all effecter/target
(E/T) cell ratios from 12.5-100 (e.g. 17 ? 3 vs. 28 ? 3% lysis without
IFN-P, P < 0.05, and 42 t 4 vs. 57 2 4% lysis with IFN-0, P < 0.05;
both at E/T 50). IGF-I levels of patients and controls showed a significant
positive correlation with NK cell activity (r = 0.37; P < 0.05).
In an IGF-I in vitro study (IGF-I in vitro 250-1250 kg/L), the basal and
IFN-P stimulated NK cell activity of 13 hGH-deficient patients and of
18 normal subjects was significantly enhanced by IGF-I in vitro (e.g.
GH-deficient patients: 9 ? 2 us. 10 2 2% lysis without IFN-P, P < 0.05
and 25 + 4 vs. 30 + 4% lysis with IFN-/3, P < 0.005; and normal
subjects: 15 + 3 vs. 23 ? 3% lysis without IFN-/3, P < 0.001 and 35 2
4 us. 44 + 5% lysis with IFN-P, P < 0.001; both at IGF-I 500 pg/L).
In summary, in our cross-sectional study, adult GH-deficient patients
showed a significantly lower basal and IFN-P stimulated NK cell
activity than matched controls, despite equal NK cell numbers. IGF-I
levels of patients and controls showed a weak positive correlation with
NK cell activity. In an in vitro study, IGF-I significantly enhanced
basal and IFN-P stimulated NK cell activity of hGH-deficient patients
and also of normal subjects. The decreased NK cell activity in GHdeficient
patients may be caused at least in part by low serum IGF-I
levels. IGF-I appears to be an independent coregulatory modulator of
NK cell activity. (Endocrinology 137: 5332-5336, 1996
Decreasing resistance in the maternal uterine and peripheral arterial system is apparently unrelated to plasma and urinary levels of nitrite/nitrate and cyclic-guanosinmonophosohate during the course of normal pregnancies
Aims: The aim of the presented study was to clarify the relationship between the pulsatility index of the uterine arteries and the maternal cubital artery and peripheral concentrations of the metabolites of nitric oxide (NO) and its second messenger cyclic guanosinmonophophate (cGMP) during the normal course of pregnancy and postpartum. Methods: 49 uncomplicated pregnancies were investigated every 46 weeks until delivery, 29 of them were additionally investigated postpartum. Paralleling each Doppler sonografic investigation maternal blood and urine samples were taken. The measurements of nitrite/ nitrate and cGMP were performed with a colorimetric and radio immuno assay. We demonstrate a significant decrease of the PI of the uterine arteries and of the cubital artery with inverse correlation to advancing gestational age. Results: The concentrations of nitrite/nitrate and cGMP remain stable during gestation and do not correlate to the PI of the uterine and cubital artery. Postpartum a reincrease in the uterine and peripheral resistance can be shown. The concentrations of urinary cGMP and nitrite/ nitrate as well as plasma cGMP remain unchanged, whereas plasma nitrite/nitrate decreases postpartum. Conclusions: The status of NO biosyntheses in normal pregnancy remains controversial. We hypothesize further systemically acting mediators which contribute to the decreasing vascular resistance
Variational calculations for the hydrogen-antihydrogen system with a mass-scaled Born-Oppenheimer potential
The problem of proton-antiproton motion in the --
system is investigated by means of the variational method. We introduce a
modified nuclear interaction through mass-scaling of the Born-Oppenheimer
potential. This improved treatment of the interaction includes the nondivergent
part of the otherwise divergent adiabatic correction and shows the correct
threshold behavior.
Using this potential we calculate the vibrational energy levels with angular
momentum 0 and 1 and the corresponding nuclear wave functions, as well as the
S-wave scattering length. We obtain a full set of all bound states together
with a large number of discretized continuum states that might be utilized in
variational four-body calculations. The results of our calculations gives an
indication of resonance states in the hydrogen-antihydrogen system
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A consensus on the diagnosis and treatment of acromegaly complications
In March 2011, the Acromegaly Consensus Group met to revise and update the guidelines on the diagnosis and treatment of acromegaly complications. The meeting was sponsored by the Pituitary Society and the European Neuroendocrinology Association and included experts skilled in the management of acromegaly. Complications considered included cardiovascular, endocrine and metabolic, sleep apnea, bone diseases, and mortality. Outcomes in selected, related clinical conditions were also considered, and included pregnancy, familial acromegaly and invasive macroadenomas. The need for a new disease staging model was considered, and design of such a tool was proposed
Calculation of the positron bound state with the copper atom
A new relativistic method for calculation of positron binding to atoms is
presented. The method combines a configuration interaction treatment of the
valence electron and the positron with a many-body perturbation theory
description of their interaction with the atomic core. We apply this method to
positron binding by the copper atom and obtain the binding energy of 170 meV (+
- 10%). To check the accuracy of the method we use a similar approach to
calculate the negative copper ion. The calculated electron affinity is 1.218
eV, in good agreement with the experimental value of 1.236 eV. The problem of
convergence of positron-atom bound state calculations is investigated, and
means to improve it are discussed. The relativistic character of the method and
its satisfactory convergence make it a suitable tool for heavier atoms.Comment: 15 pages, 5 figures, RevTe
Configuration-interaction calculations of PsH and e(+)Be
The configuration-interaction (CI) method is applied to the study of the positronium-hydride (PsH) and positronic-beryllium (e+Be) systems. The binding energy and other properties are slowly convergent with respect to the angular momentum of the orbitals used to construct the CI basis states. The largest calculations recover 94% and 80% of the binding energy against dissociation when compared with existing calculations of PsH and e+ Be. Extrapolating using Cl convergence trends improves these results to 99% and 98%, respectively. Convergence is not so good for the electron-positron annihilation rates, but the extrapolated annihilation rates were within 10% of the best calculations. Two different schemes have been used to construct the CI basis, and it is found that it is possible to discard roughly half the CI basis with almost no degradation in the binding energy and the annihilation rate. These investigations demonstrate the feasibility of using single particle orbitals centred on the nucleus to represent positronic systems with two valence electrons
Object Detection Through Exploration With A Foveated Visual Field
We present a foveated object detector (FOD) as a biologically-inspired
alternative to the sliding window (SW) approach which is the dominant method of
search in computer vision object detection. Similar to the human visual system,
the FOD has higher resolution at the fovea and lower resolution at the visual
periphery. Consequently, more computational resources are allocated at the
fovea and relatively fewer at the periphery. The FOD processes the entire
scene, uses retino-specific object detection classifiers to guide eye
movements, aligns its fovea with regions of interest in the input image and
integrates observations across multiple fixations. Our approach combines modern
object detectors from computer vision with a recent model of peripheral pooling
regions found at the V1 layer of the human visual system. We assessed various
eye movement strategies on the PASCAL VOC 2007 dataset and show that the FOD
performs on par with the SW detector while bringing significant computational
cost savings.Comment: An extended version of this manuscript was published in PLOS
Computational Biology (October 2017) at
https://doi.org/10.1371/journal.pcbi.100574
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